dc.contributor.author | Orikiriza, Patrick | |
dc.contributor.author | Smith, Julianna | |
dc.contributor.author | Ssekyanzi, Bob | |
dc.contributor.author | Nyehangane, Dan | |
dc.contributor.author | Taremwa, Ivan Mugisha | |
dc.contributor.author | Turyashemererwa, Esther | |
dc.contributor.author | Byamukama, Onesmas | |
dc.contributor.author | Tusabe, Tobias | |
dc.contributor.author | Ardizzoni, Elisa | |
dc.contributor.author | Marais, Ben J | |
dc.contributor.author | Wobudeya, Eric | |
dc.contributor.author | Kemigisha, Elizabeth | |
dc.contributor.author | Amumpaire, Juliet Mwanga | |
dc.contributor.author | Nampijja, Dora | |
dc.contributor.author | Bonnet, Maryline | |
dc.date.accessioned | 2022-01-14T08:55:09Z | |
dc.date.available | 2022-01-14T08:55:09Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Orikiriza, P., Smith, J., Ssekyanzi, B., Nyehangane, D., Taremwa, I. M., Turyashemererwa, E., ... & Bonnet, M. (2022). Tuberculosis diagnostic accuracy of stool Xpert MTB/RIF and urine AlereLAM in vulnerable children. European Respiratory Journal, 59(1). | en_US |
dc.identifier.uri | http://ir.must.ac.ug/xmlui/handle/123456789/1174 | |
dc.description | This is an article. | en_US |
dc.description.abstract | Background Non-sputum-based diagnostic approaches are crucial in children at high risk of disseminated
tuberculosis (TB) who cannot expectorate sputum. We evaluated the diagnostic accuracy of stool Xpert
MTB/RIF and urine AlereLAM tests in this group of children.
Methods Hospitalised children with presumptive TB and either age <2 years, HIV-positive or with severe
malnutrition were enrolled in a diagnostic cohort. At enrolment, we attempted to collect two urine, two
stool and two respiratory samples. Urine and stool were tested with AlereLAM and Xpert MTB/RIF,
respectively. Respiratory samples were tested with Xpert MTB/RIF and mycobacterial culture. Both a
microbiological and a composite clinical reference standard were used.
Results The study analysed 219 children; median age 16.4 months, 72 (32.9%) HIV-positive and 184
(84.4%) severely malnourished. 12 (5.5%) and 58 (28.5%) children had confirmed and unconfirmed TB,
respectively. Stool and urine were collected in 219 (100%) and 216 (98.6%) children, respectively. Against
the microbiological reference standard, the sensitivity and specificity of stool Xpert MTB/RIF was 50.0%
(6/12, 95% CI 21.1–78.9%) and 99.1% (198/200, 95% 96.4–99.9%), while that of urine AlereLAM was
50.0% (6/12, 95% 21.1–78.9%) and 74.6% (147/197, 95% 67.9–80.5%), respectively. Against the
composite reference standard, sensitivity was reduced to 11.4% (8/70) for stool and 26.2% (17/68) for
urine, with no major difference by age group (<2 and ⩾2 years) or HIV status.
Conclusions The Xpert MTB/RIF assay has excellent specificity on stool, but sensitivity is suboptimal.
Urine AlereLAM is compromised by poor sensitivity and specificity in children | en_US |
dc.description.sponsorship | This study was funded by Médecins Sans Frontières. Funding information for this article has
been deposited with the Crossref Funder Registry | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | EUROPEAN RESPIRATORY JOURNAL | en_US |
dc.title | Tuberculosis diagnostic accuracy of stool Xpert MTB/RIF and urine AlereLAM in vulnerable children | en_US |
dc.type | Article | en_US |