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dc.contributor.authorAmpaire, Lucas M.
dc.contributor.authorKatawera, Victoria
dc.contributor.authorNyehangane, Dan
dc.contributor.authorBoum, Yap
dc.contributor.authorBazira, Joel
dc.date.accessioned2022-04-25T09:29:43Z
dc.date.available2022-04-25T09:29:43Z
dc.date.issued2015
dc.identifier.citationAmpaire, L. M., Katawera, V., Nyehangane, D., Boum, Y., & Bazira, J. (2015). Epidemiology of carbapenem resistance among multi-drug resistant enterobacteriaceae in Uganda. British microbiology research journal, 8(2), 418.en_US
dc.identifier.urihttp://ir.must.ac.ug/xmlui/handle/123456789/1824
dc.description.abstractBackground: Multi-drug resistant (MDR) Enterobacteriaceae are on the increase worldwide and their spread has become a global challenge. Escalating the challenge is the possibility that many of these are Carbapenemase-producing Enterobacteriaceae (CPE). This further complicates patient management. The magnitude of MDR-CPE in many developed settings has been reported, however, there is paucity of data from resource limited settings. We evaluated the epidemiology of MDR-CPE of clinical origin in South Western Uganda. Methods: From September 2013 to June 2014, all Enterobacteriaceae isolated from diverse specimens obtained from patients attending Mbarara Regional Referral Hospital, South-western Uganda, were screened for MDR in a laboratory-based cross sectional study. Isolates found to be MDR were screened for carbapenem susceptibility/resistance phenotypically by Kirby Bauer disc diffusion method following CLSI guidelines and genetically using the multiplex real-time Polymerase Chain Reaction (RT-PCR). Results: Of the 658 strains isolated, 183 (27.8%) were MDR and 68 (37.15%) of those MDR exhibited at least one form of carbapenem resistance with 23 (12.57%) and 56 (30.60%) isolates expressing phenotypic and genetic resistance, respectively. Eleven MDR-CPE (6.01%) isolates exhibited both phenotypic and genotypic resistance to carbapenems. Only blaVIM and blaOXA-48 genes were detected among the genetically resistant isolates. Conclusion: The high prevalence of MDR-CPE calls for aggressive infection control and prevention strategies, including reinforcement of hand hygiene, using contact precautions and early detection of CPE through use of targeted surveillance and molecular techniques in resource limited settings.en_US
dc.description.sponsorshipMEPI-MESAU Grant Number 5R24TW008886 supported by OGAC, NIH and HRSA.en_US
dc.language.isoen_USen_US
dc.publisherBritish Microbiology Research Journalen_US
dc.subjectEnterobacteriaceaeen_US
dc.subjectCarbapenemaseen_US
dc.subjectMulti-drug resistanten_US
dc.subjectCPEen_US
dc.subjectESBLen_US
dc.titleEpidemiology of Carbapenem Resistance among Multi-drug Resistant Enterobacteriaceae in Ugandaen_US
dc.typeArticleen_US


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