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dc.contributor.authorYang, Haechan J.
dc.contributor.authorWasswa, William
dc.contributor.authorLi, Raymond
dc.contributor.authorPautler, Brandon
dc.contributor.authorFry, Aiden
dc.contributor.authorLeung, Rajan
dc.contributor.authorHoldsworth, David W.
dc.contributor.authorCunningham, Ian A.
dc.date.accessioned2024-11-26T08:39:34Z
dc.date.available2024-11-26T08:39:34Z
dc.date.issued2024
dc.identifier.citationYang, H. J., Wasswa, W., Li, R., Pautler, B., Fry, A., Leung, R., ... & Cunningham, I. A. (2024, March). Optical resolution and MTF of a low-cost Fourier ptychography microscope using a raspberry pi computer. In Optics and Biophotonics in Low-Resource Settings X (Vol. 12832, pp. 19-27). SPIE.en_US
dc.identifier.urihttp://ir.must.ac.ug/xmlui/handle/123456789/3954
dc.description.abstractIntroduction: Low-cost optical microscopes are under development by several groups to bring point-of-care malaria diagnosis services to under-served communities in low-resource settings. We describe a Fourier ptychography microscope (FPM) system based on an open-source design and a method of measuring spatial resolution in terms of the optical modulation transfer function (MTF) using images of the 1951 USAF resolution test device. Methods: The FPM system uses a Raspberry Pi computer and 196-LED matrix light source. FPM images were reconstructed using in-house Python code. The MTF was determined from bar-patterns using a least-squares analysis to fit a square wave, including odd-harmonic terms, to image profiles through the bars. These were normalized to larger uniform regions of the pattern and combined to generate the MTF. Results: Total component cost of the FPM was less than $200. The theoretical diffraction limit imposed by the pupil function of this system was 280 cycles/mm, slightly less than the measured MTF 10% frequency of 300 cycles/mm. The 10% frequency in FPM images was 550 cycles/mm. Conclusions: The USAF test pattern provides a practical method for assessing FPM performance in terms of the achieved MTF of the $200 Raspberry Pi based Fourier ptychography microscope. The limiting frequency in FPM images, 550 cycles/mm, was slightly less than the Nyquist sampling cut-off frequency of 670 cycles/mm imposed by pixel spacing.en_US
dc.description.sponsorshipWestern University Frugal Biomedical Innovation programen_US
dc.language.isoen_USen_US
dc.publisherIn Optics and Biophotonics in Low-Resource Settings Xen_US
dc.subjectMalariaen_US
dc.subjectFourier Ptychography Microscopeen_US
dc.subjectComputeren_US
dc.titleOptical Resolution and MTF of a Low-Cost Fourier Ptychography Microscope using a Raspberry Pi Computeren_US
dc.typeArticleen_US


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