dc.contributor.author | Atwebembeire, J. | |
dc.contributor.author | Orikiriza, P. | |
dc.contributor.author | Bonnet, M. | |
dc.contributor.author | Atwine, D. | |
dc.contributor.author | Katawera, V. | |
dc.contributor.author | Nansumba, M. | |
dc.contributor.author | Nyehangane, D. | |
dc.contributor.author | Bazira, J. | |
dc.contributor.author | Amumpaire, J. Mwanga | |
dc.contributor.author | Byarugaba, F. | |
dc.contributor.author | II, Y. Boum | |
dc.date.accessioned | 2022-05-25T13:42:26Z | |
dc.date.available | 2022-05-25T13:42:26Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Atwebembeire, J., Orikiriza, P., Bonnet, M., Atwine, D., Katawera, V., Nansumba, M., ... & Boum, Y. (2016). Xpert® MTB/RIF for detection of Mycobacterium tuberculosis from frozen string and induced sputum sediments. The International Journal of Tuberculosis and Lung Disease, 20(8), 1113-1117. | en_US |
dc.identifier.uri | http://ir.must.ac.ug/xmlui/handle/123456789/2049 | |
dc.description.abstract | Settings: Although it is now widely used for tuberculosis (TB) diagnosis, Xpertw MTB/RIF availability remains inadequate in low-resource settings. Moreover, its accuracy in testing stored samples from nonexpectorating patients has not been evaluated.
Objective: To assess the performance of Xpert in frozen samples of induced sputum (IS) and sputum from string test (ST) from non-expectorating individuals with presumed TB.
Design: This was a laboratory-based study of 377 ST and IS samples collected between March 2010 and March 2013 at a referral hospital in Uganda. Samples were decontaminated, centrifuged and cultured, and the resultant samples were frozen at _208C before Xpert evaluation.
Results: TB was detected in ST and IS samples from 19/163 (11.7%) children and 63/201 (29.4%) adults using culture. Xpert sensitivity in frozen sediments from children was 37.5% (95%CI 8.5–75.5) in STand 41.7% (95%CI 15.2–72.3) in IS samples, with specificities of respectively 100% (95%CI 94.9–100) and 98.6% (95%CI 92.7–100). In adults, sensitivity was respectively 50% (95%CI 31.3–68.7) and 48.5% (95%CI 30.8– 66.4) in ST and IS samples, with specificities of respectively 100% (95%CI 95.5–100) and 98.6% (95%CI 92.4–100).
Conclusion: Given these results, and particularly the high specificity, the use of Xpert on frozen ST and IS sediment samples from both children and adults is promising. | en_US |
dc.description.sponsorship | Medical Education for Equitable Services to All Ugandans (Mbarara, Uganda), a Medical Education Partnership Initiative (Grant no 5R24TW008886) from the Office of the US Global AIDS Coordinator and the US Department of Health and Human Services (Washington DC, USA), Health Resources and Services Administration (Rockville, MD, USA) and National Institutes of Health (Bethesda, MD, USA). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | The International Journal of Tuberculosis and Lung Disease | en_US |
dc.subject | TB diagnosis | en_US |
dc.subject | String test | en_US |
dc.subject | Induced sputum | en_US |
dc.subject | Non-expectorating | en_US |
dc.subject | Xpert | en_US |
dc.title | XpertW MTB/RIF for detection of Mycobacterium tuberculosis from frozen string and induced sputum sediments | en_US |
dc.type | Article | en_US |