dc.contributor.author | Ezeonwumelu, Joseph Obiezu Chukwujekwu | |
dc.contributor.author | Ntale, Muhammad | |
dc.contributor.author | Kasozi, Keneth Iceland | |
dc.contributor.author | Ogbonnia, Steve Okwudili | |
dc.contributor.author | Tanayen, Julius Kihdze | |
dc.contributor.author | Agwu, Ezera | |
dc.contributor.author | Okonkwo, Chukwudi Onyeka | |
dc.contributor.author | Akunne, Ambrose Amamchukwu | |
dc.contributor.author | Byarugaba, Frederick | |
dc.date.accessioned | 2022-02-11T09:37:53Z | |
dc.date.available | 2022-02-11T09:37:53Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Ezeonwumelu, J. O. C., Muhammad, N., Iceland, K. K., Ogbonnia, S. O., Tanayen, J. K., Agwu, E., ... & Byarugaba, F. (2016). Resistance, Minimum Inhibitory and Bactericidal Concentration Profiles of Oral Bacteria from HIV/AIDS Patients in South Western Uganda. Br J Med Med Res, 18(11), 1-14. | en_US |
dc.identifier.uri | http://ir.must.ac.ug/xmlui/handle/123456789/1464 | |
dc.description.abstract | Background: The development of drug resistance is a major challenge in the management of
microbial infections especially in immune-compromised (HIV/AIDS) patients.
Objective: This was to assess levels of antibacterial resistance; minimum inhibitory and bactericidal profiles of oral bacteria isolated from HIV/ AIDS patients in South Western Uganda and compare their levels with those of the reference organisms (control).
Methods: Bacterial isolates were grown on Mueller Hinton Agar, and biochemical tests were
conducted using conventional and analytical profile index 20 sugar panel methods to identify
strains. Antibiograms using modified Kirby-Bauer tube dilution and agar well diffusion methods
were performed on purified isolates using antibiotic discs for resistance analysis and E-test strips
for MIC and MBC analysis. Data were analysed using ANOVA with p< 0.05 considered statistically significant.
Results: All the tested bacteria except Salmonella pullorum and non haemolytic streptococcus
showed 50 to 100% resistance to cotrimoxazole and erythromycin demonstrating resistance
development in HIV/AIDS patients in rural communities of Uganda against commonly used
antibacterials for management of opportunistic infections. Staphylococcus aureus and Escherichia
coli was both ˃60% resistant to cotrimoxazole. Pseudomonas aeruginosa and Bacillus cereus
were absolutely resistant (100%) to all the antibacterial agents used in this study. MIC and MBC
levels for S. aureus when compared with S. aureus ATCC 25293 were highly related showing the
level of ineffectiveness of the tested drugs (p=0.235>0.05 (MIC) and p=0.409>0.05 (MBC). High
MIC and MBC levels of cotrimoxazole against Pseudomonas aeruginosa were followed by those of Staphylococcus aureus, perhaps associated with neutropenia and granulocyte dysfunction in
human infections, necessitating appropriate dosage adjustments. Gentamycin and ceftriaxone had
high MIC and MBC levels against E. coli respectively. Further analysis showed significance in
ciprofloxacin against all the bacteria in its low MICs.
Conclusion: Bacterial resistance and poor drug efficacy in HIV/AIDS patients in rural communities are a major challenge in Uganda | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | British Journal of Medicine & Medical Research | en_US |
dc.subject | Bactericidal concentration | en_US |
dc.subject | Bacterial inhibition | en_US |
dc.subject | Drug resistance | en_US |
dc.subject | HIV/AIDS in Uganda | en_US |
dc.title | Resistance, Minimum Inhibitory and Bactericidal Concentration Profiles of Oral Bacteria from HIV/AIDS Patients in South Western Uganda | en_US |
dc.type | Article | en_US |