Modified Plasma-Thrombin Method of Cell Block Preparation for Fine-Needle Aspiration Biopsies in Resource-Limited Settings
dc.contributor.author | Aisagbonhi, Omonigho | |
dc.contributor.author | Birungi, Abraham | |
dc.contributor.author | Atwine, Raymond | |
dc.contributor.author | Behayo, Paddy | |
dc.contributor.author | Ayebaziwe, Benon | |
dc.contributor.author | Roberts, Drucilla | |
dc.contributor.author | Tambouret, Rosemary | |
dc.date.accessioned | 2022-05-17T09:51:11Z | |
dc.date.available | 2022-05-17T09:51:11Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Aisagbonhi, O., Birungi, A., Atwine, R., Behayo, P., Ayebaziwe, B., Roberts, D., & Tambouret, R. (2018). Modified plasma-thrombin method of cell block preparation for fine-needle aspiration biopsies in resource-limited settings. American Journal of Clinical Pathology, 150(2), 137-145. | en_US |
dc.identifier.uri | http://ir.must.ac.ug/xmlui/handle/123456789/1940 | |
dc.description.abstract | Objectives: The plasma-thrombin method is commonly used to make cell blocks from fine-needle aspiration (FNA) samples but requires centrifugation. We describe a modification to this method that does not require centrifugation for use in resource-limited settings. Methods: Pooled fresh plasma is aliquoted into 2-mL Eppendorf tubes and the FNA sample directly rinsed into the plasma. Two drops of reconstituted thrombin are added and gently mixed. A cell clot is transferred to a tissue bag, fixed in formalin, and processed. This method was applied to FNA samples from 44 patients presenting to the Mbarara University of Science and Technology FNA clinic. Results: The cell blocks were less cellular than the smears but contained adequate material to confirm morphologic impression or perform immunocytochemistry in 36 of 44 cases (82% adequacy rate). Conclusions: The modified plasma-thrombin method is a reliable cell block preparation method that can be easily applied in resource-limited settings. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | American Journal of Clinical Pathology | en_US |
dc.subject | Fine-needle aspiration | en_US |
dc.subject | Cell block | en_US |
dc.subject | Plasma-thrombin | en_US |
dc.subject | Resource limited | en_US |
dc.title | Modified Plasma-Thrombin Method of Cell Block Preparation for Fine-Needle Aspiration Biopsies in Resource-Limited Settings | en_US |
dc.type | Article | en_US |
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