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dc.contributor.authorKatawera, Victoria
dc.contributor.authorSiedner, Mark
dc.contributor.authorII, Yap Boum
dc.date.accessioned2023-11-17T06:57:39Z
dc.date.available2023-11-17T06:57:39Z
dc.date.issued2014
dc.identifier.citationKatawera, V., Siedner, M., & Boum Ii, Y. (2014). Evaluation of the modified colorimetric resazurin microtiter plate-based antibacterial assay for rapid and reliable tuberculosis drug susceptibility testing. BMC microbiology, 14, 1-4.en_US
dc.identifier.urihttp://ir.must.ac.ug/xmlui/handle/123456789/3276
dc.description.abstractBackground: The resazurin microtiter assay (classic REMA), a colorimetric liquid culture-based drug susceptibility assay for Mycobacterium tuberculosis (MTB), has been endorsed by the World Health Organization. The assay requires 8-16 days to obtain results, delaying management of drug resistant tuberculosis patients. A modified REMA which allows results in as little as 24 hours for bacterial strains, has been developed and validated using Staphylococcus aureus, but has not yet been evaluated for MTB. Therefore, we assessed the performance of the modified REMA for rifampicin (RIF) and isoniazid (INH) susceptibility, using the classic REMA as the reference standard. We also compared simplicity (from the technicians’ point of view), time taken to obtain results (rank-sum testing), specificity and Kappa statistics of the two methods. Results: The modified REMA, which is a one-step procedure, was found to be simpler to perform and results were obtained in a significantly shorter time (5 versus 9 days, p < 0.0001) compared to the classic REMA due to addition of indicator and strain at the same time. The specificity of the modified REMA was low {46.8% (35.5% - 58.4%) for RIF and 13.9% (7.2% - 23.5%) for INH}. Kappa statistics were 16.0% for RIF and 2.0% for INH. Low specificity and kappa statistics are due to indicator reduction by the strains before complete drug activity. Conclusion: Although modified REMA is faster and simpler compared to classic REMA, it is not reliable for MTB drug susceptibility testing.en_US
dc.description.sponsorshipBelgian Embassy Development Agency, Uganda and The Council for Frontiers of Knowledge (The CFK).en_US
dc.language.isoen_USen_US
dc.publisherBMC microbiologyen_US
dc.subjectResazurin microtiter assayen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectTuberculosisen_US
dc.subjectDrug testingen_US
dc.titleEvaluation of the modified colorimetric resazurin microtiter plate-based antibacterial assay for rapid and reliable tuberculosis drug susceptibility testingen_US
dc.typeArticleen_US


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