dc.contributor.author | Devaud, L. Leslie | |
dc.contributor.author | Alele, Paul | |
dc.date.accessioned | 2020-01-22T13:20:08Z | |
dc.date.available | 2020-01-22T13:20:08Z | |
dc.date.issued | 2004-06 | |
dc.identifier.citation | Differential effects of chronic ethanol administration and withdrawal on γ‐aminobutyric acid type A and NMDA receptor subunit proteins in male and female rat brain LL Devaud, P Alele - Alcoholism: Clinical and Experimental …, 2004 - Wiley Online Library Background: Investigations have shown that chronic ethanol exposure results in selective alterations in levels of γ‐aminobutyric acid (GABA) A and NMDA receptor subunits. We previously reported significant sex differences in these chronic ethanol‐induced adaptations. Because we have more recently found important sex differences in timing for the development of and recovery from ethanol dependence, we wanted to ascertain whether there were associations between overt expression of withdrawal and neuroadaptations at | en_US |
dc.identifier.uri | http://ir.must.ac.ug/xmlui/handle/123456789/441 | |
dc.description.abstract | Background: Investigations have shown that chronic ethanol exposure results in selective alterations in
levels of -aminobutyric acid (GABA)A and NMDA receptor subunits. We previously reported significant
sex differences in these chronic ethanol-induced adaptations. Because we have more recently found important sex differences in timing for the development of and recovery from ethanol dependence, we wanted
to ascertain whether there were associations between overt expression of withdrawal and neuroadaptations
at the level of GABAA and NMDA receptors.
Methods: Western blot analysis was used to assay protein levels for several GABAA and NMDA
receptor subunits in rat cerebral cortex and hippocampus by using subunit-selective antibodies. Rats were
fed 6% ethanol in a liquid diet with pair-fed controls. Feeding, harvesting of tissue, and Western blot
experiments were all conducted while maintaining the paired design. Tissue was harvested after 3 days of
ethanol exposure, 9 days of ethanol exposure, or 3 days of ethanol withdrawal after 14 days of liquid diet
administration.
Results: We again found sex-, subunit-, and brain region–selective effects of ethanol administration and
withdrawal for GABAA and NMDA receptors. There was a strong association between increased GABAA
receptor 4 subunit levels and previously determined withdrawal-induced changes in seizure susceptibility,
highlighted by the sex differences in ethanol exposure length required to cause withdrawal signs. In
addition, results obtained after 9 days of ethanol administration were in general agreement with previous
findings after 14 days of ethanol administration.
Conclusions: These data further support the suggestion that alterations in subunit assembly of GABAA
and NMDA receptors may have some mechanistic role in neuroadaptations underlying ethanol dependence and withdrawal. Furthermore, significant sex differences in these adaptations suggest that multiple
types of adaptations may be elicited, depending on innate differences in the actions/effects of ethanol.
Key Words: Ethanol Dependence, Ethanol Withdrawal, GABA, Glutamate, Sex Differences. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartofseries | ;Vol 28, No 6, 2004: pp 957–965 | |
dc.subject | Ethanol Dependence | en_US |
dc.subject | Ethanol Withdrawal | en_US |
dc.subject | GABA | en_US |
dc.subject | Glutamate | en_US |
dc.subject | Sex Differences. | en_US |
dc.title | Differential Effects of Chronic Ethanol Administration and Withdrawal on -Aminobutyric Acid Type A and NMDA Receptor Subunit Proteins in Male and Female Rat Brain | en_US |
dc.type | Article | en_US |