dc.contributor.author | Okafor, Elizabeth C. | |
dc.contributor.author | Hullsiek, Katherine H | |
dc.contributor.author | Williams, Darlisha A. | |
dc.contributor.author | Scriven, James E. | |
dc.contributor.author | Rhein, Joshua | |
dc.contributor.author | Nabeta, Henry W. | |
dc.contributor.author | Musubire, Abdu K. | |
dc.contributor.author | Rajasingham, Radha | |
dc.contributor.author | Muzoora, Conrad | |
dc.contributor.author | Schutz, Charlotte | |
dc.contributor.author | Meintjes, Graeme | |
dc.contributor.author | Meya, David B. | |
dc.contributor.author | Boulware, David R. | |
dc.date.accessioned | 2022-06-13T10:16:32Z | |
dc.date.available | 2022-06-13T10:16:32Z | |
dc.date.issued | 2020-10-29 | |
dc.identifier.citation | Okafor, E. C., Hullsiek, K. H., Williams, D. A., Scriven, J. E., Rhein, J., Nabeta, H. W., ... & Boulware, D. R. (2020). Correlation between blood and CSF compartment cytokines and chemokines in subjects with cryptococcal meningitis. Mediators of inflammation, 2020. | en_US |
dc.identifier.uri | http://ir.must.ac.ug/xmlui/handle/123456789/2100 | |
dc.description.abstract | Background: Though peripheral blood is a crucial sample to study immunology, it is unclear whether the immune environment in the peripheral vasculature correlates with that at the end-organ site of infection. Using cryptococcal meningitis as a model, we investigated the correlation between serum and cerebrospinal fluid biomarkers over time.
Methods: We analyzed the cerebrospinal fluid and serum of 160 subjects presenting with first episode cryptococcal meningitis for soluble cytokines and chemokines measured by Luminex assay. Specimens were collected at meningitis diagnosis, 1-week, and 2-week post cryptococcal diagnosis. We compared paired samples by Spearman’s correlation and the p value was set at <0.01.
Results: Of the 21 analytes tested at baseline, there was no correlation detected between nearly all analytes. A weak negative correlation was found between serum and cerebrospinal fluid levels of interferon-gamma (Rho = −0:214; p = :007) and interleukin-4 (Rho = −0:232; p = :003). There was no correlation at 1-week post cryptococcal diagnosis. However, at 2-week post cryptococcal
diagnosis, there was a weak positive correlation of granulocyte-macrophage colony-stimulating factor levels (Rho = 0:25; p = :007) in serum and cerebrospinal fluid. No cytokine or chemokine showed consistent correlation overtime.
Conclusion: Based on our analysis of 21 biomarkers, serum and cerebrospinal fluid immune responses do not correlate. There appears to be a distinct immune environment in terms of soluble biomarkers in the vasculature versus end-organ site of infection. While this is a model of HIV-related cryptococcal meningitis, we postulate that assuming the blood compartment is representative of the immune function at the end-organ site of infection may not be appropriate. | en_US |
dc.description.sponsorship | National Institute of Allergy and Infectious Diseases (U01AI125003, T32AI055433, and K23AI138851), National Institute of Neurological Disorders and Stroke (R01NS086312), and Fogarty International Center (R01NS086312, K01TW010268, and K43TW010718). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Mediators of Inflammation | en_US |
dc.subject | Peripheral blood | en_US |
dc.subject | CSF Compartment Cytokines | en_US |
dc.subject | Cryptococcal meningitis | en_US |
dc.subject | Cerebrospinal fluid biomarkers | en_US |
dc.title | Correlation between Blood and CSF Compartment Cytokines and Chemokines in Subjects with Cryptococcal Meningitis | en_US |
dc.type | Article | en_US |