dc.contributor.author | J, Bazira | |
dc.contributor.author | M, Matte | |
dc.contributor.author | BB, Asiimwe | |
dc.contributor.author | LM, Joloba | |
dc.date.accessioned | 2022-05-16T08:38:12Z | |
dc.date.available | 2022-05-16T08:38:12Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Bazira, J., Matte, M., Asiimwe, B. B., & Joloba, L. M. (2010). Genetic diversity of mycobacterium tuberculosis in Mbarara, South Western Uganda. African health sciences, 10(4). | en_US |
dc.identifier.uri | http://ir.must.ac.ug/xmlui/handle/123456789/1926 | |
dc.description.abstract | Background: We determined the genetic diversity of mycobacteria isolated from tuberculosis patients in Mbarara Uganda, using region of difference (RD) analysis and spacer oligonucleotide typing (spoligotyping).
Methods: Sputum samples were cultured on Lowenstein Jensen media. The isolates were characterized using RD analysis and spoligotyping.
Results: The majority (92.8%) of the patients were new cases, 60% were males and 44% were HIV positive with a mean age of 33.7 years. All the 125 isolates were identified as M.tuberculosis sensu stricto. Most (92.8%) of the isolates were modern strains. Spoligotyping revealed 79 spoligotype patterns, with an overall diversity of 63.2%. Sixty (48%) isolates formed 16 clusters each consisting of 2-15 isolates. Mst (59.2 %) of the isolates were Uganda genotype strains. The major shared spoligotypes in our sample were SIT 135 (T2-Uganda) with 12 isolates and SIT 128 (T2) with 5 isolates. Sixty-nine (87%) patterns had not yet been defined in the SpolDB4.0. database.
Conclusion: The TB epidemic in Mbarara is caused mainly by modern M-Tuberculosis strains of the Uganda genotype. The wide diversity of strains may indicate that the majority of the TB cases are reactivation rather than re-infection. However, this needs to be ascertained with more discriminative finger printing technique | en_US |
dc.description.sponsorship | DAAD and WHO/Tropical disease research. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | African health sciences | en_US |
dc.subject | Mycobacteria | en_US |
dc.subject | Spoligotypes | en_US |
dc.subject | Region of difference | en_US |
dc.title | Genetic diversity of Mycobacterium tuberculosis in Mbarara, South Western Uganda | en_US |
dc.type | Article | en_US |