dc.contributor.author | Petra, Nalumaga Pauline | |
dc.contributor.author | Mukasa, James Kiguli | |
dc.contributor.author | Musinguzi, Benson | |
dc.contributor.author | Busingye, Jude Collins | |
dc.contributor.author | Muasya, Daniel Wambua | |
dc.contributor.author | Njovu, Israel Kiiza | |
dc.contributor.author | Birungi, Abraham | |
dc.contributor.author | Kassaza, Kennedy | |
dc.contributor.author | Bazira, Joel | |
dc.contributor.author | Itabangi, Herbert | |
dc.date.accessioned | 2024-11-26T07:29:48Z | |
dc.date.available | 2024-11-26T07:29:48Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Petra, N. P., Mukasa, J. K., Musinguzi, B., Busingye, J. C., Muasya, D. W., Njovu, I. K., ... & Itabangi, H. (2024). Detection of Mutated erg11 and fks1 Genes among Resistant Candida Species Isolated in Pregnant Women in Mbarara, Uganda. International STD Research & Reviews, 13(2), 57-66. | en_US |
dc.identifier.uri | http://ir.must.ac.ug/xmlui/handle/123456789/3950 | |
dc.description.abstract | Background: Vulvovaginal candidiasis is estimated to range between 35-60% among pregnant women worldwide. The emergency of anti-fungal resistance in Candida species against azoles and caspofungin is a rising concern because there is a limited range of choices of antifungals to be used in pregnant women with low toxicity. In Uganda, the burden of vulvovaginal candidiasis is estimated to be 48%. However, there is limited data regarding in vitro phenotypic and genotypic anti-fungal susceptibility patterns among candida species isolated from pregnant women. Thus, this study aimed to screen for the two mutated famous erg11 and fks1 genes that lead to anti-fungal resistance among clinical candida isolates.
Methods: A cross-sectional study involving 90 Candida species isolates previously collected from a larger study carried out from Mbarara regional referral hospital. Phenotypic susceptibility methods (Kirby-Bauer and minimum inhibitory concentration) while Polymerase chain reaction method and gel electrophoresis were used for detection of the amplified mutated ERG11 and FKS1 genes. Mean and chi-square tests were used to evaluate the associations of resistance patterns between resistant and susceptible isolates.
Results: Out of the 90 Candida isolates recovered, 56% were Candida albicans, C.glabrata were 31.11% (28/90), C. parapsilosis and C.famata accounted for 4.44% (4/90) each, C. krusei accounted for 3.33% (3/90) while C. tropicalis accounted for 1.11% (1/90). All the Candida isolates were susceptible to caspofungin while fluconazole resistance was 34.4%. The FKS1 mutated gene was not detected in randomly selected caspofungin susceptible isolates. The ERG11 mutated gene was detected in 80.6% of the fluconazole-resistant isolates and 87.5% of the isolates with intermediate activity towards fluconazole.
Conclusion: This study provides evidence that mutated erg11 gene causes reduced fluconazole drug susceptibility (p-value 0.001). Susceptible dose dependence should not be ignored as it may be associated with ERG11 gene mutation leading to resistance to fluconazole. | en_US |
dc.description.sponsorship | European Developing Countries Clinical Trails Partnership (EDCTP) with support from the European Union (Grant Ref: TMA 2019 CDF- 2789) | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | International STD Research & Reviews | en_US |
dc.subject | erg11 | en_US |
dc.subject | fks1 | en_US |
dc.subject | azoles | en_US |
dc.subject | echinocandins | en_US |
dc.subject | Candida species | en_US |
dc.title | Detection of Mutated erg11 and fks1 Genes among Resistant Candida Species Isolated in Pregnant Women in Mbarara, Uganda | en_US |
dc.type | Article | en_US |