Use of the GenoType® MTBDRplus assay to assess drug resistance of Mycobacterium tuberculosis isolates from patients in rural Uganda
![Thumbnail](/xmlui/bitstream/handle/123456789/1358/Use%20of%20the%20GenoType%c2%ae%20MTBDR%20plus%20assay%20to%20assess%20drug%20resistance%20of%20Mycobacterium%20tuberculosis%20isolates%20from%20patients%20in%20rural%20Uganda.pdf.jpg?sequence=4&isAllowed=y)
View/ Open
Date
2010-08-06Author
Bazira, Joel
Asiimwe, Benon B
Joloba, Moses L
Bwanga, Fred
Matee, Mecky I
Metadata
Show full item recordAbstract
Background: Drug resistance levels and patterns among Mycobacterium tuberculosis isolates from newly diagnosed and previously treated tuberculosis patients in Mbarara Uganda were investigated.
Methods: We enrolled, consecutively, all newly diagnosed and previously treated smear-positive TB patients aged ≥ 18 years. Isolates were tested for drug resistance against rifampicin (RIF) and isoniazid (INH) using the Genotype® MDRTBplus assay and results were compared with those obtained by the indirect proportion method on Lowenstein-Jensen media. HIV testing was performed using two rapid HIV tests.
Results: A total of 125 isolates from 167 TB suspects with a mean age 33.7 years and HIV prevalence of 67.9% (55/81) were analysed. A majority (92.8%) of the participants were newly presenting while only 7.2% were retreatment cases. Resistance mutations to either RIF or INH were detected in 6.4% of the total isolates. Multidrug resistance, INH and RIF resistance was 1.6%, 3.2% and 4.8%, respectively. The rpob gene mutations seen in the sample were D516V, S531L, H526Y H526 D and D516V, while one strain had a Δ1 mutation in the wild type probes. There were three strains with katG (codon 315) gene mutations while only one strain showed the inhA promoter region gene mutation.
Conclusion: The TB resistance rate in Mbarara is relatively low. The GenoType® MTBDRplus assay can be used for rapid screening of MDR-TB in this setting
Collections
- Research Articles [97]